Journal article
A CAF01-adjuvanted whole asexual blood-stage liposomal malaria vaccine induces a CD4 T-cell-dependent strain-transcending protective immunity in rodent models
WA Okoth, MF Ho, M Zaman, E Cooper, P Som, M Burgess, M Walton, RJ Nevagi, L Beattie, D Murphy, DI Stanisic, MF Good
Mbio | AMER SOC MICROBIOLOGY | Published : 2023
Abstract
Malaria is a leading cause of illness and death in children under 5 years of age in Sub-Saharan Africa. Currently, there is no highly efficacious malaria vaccine capable of inducing long-lasting immunity. This has increased interest in exploring different vaccine development strategies, including whole-parasite vaccines, and utilizing more effective adjuvant delivery systems. Here, we evaluate the immunogenicity and protective efficacy of a whole-parasite Plasmodium yoelii 17X blood-stage vaccine formulated with the clinically tested cationic adjuvant formulation, CAF01. The vaccine protected both inbred and outbred mice, and protected mice from homologous and heterologous challenge infectio..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
We gratefully acknowledge Jelena Vider for providing guidance on flow cytometry analyses for the red blood cell work, Miles Davenport and David Khoury for providing advice on tracking red blood cells, Statens Serum Institute for providing CAF01 used in the study comparing i.m. versus s.c. routes of vaccine administration, Hamish McMath, and animal facility staff at Griffith University.r We acknowledge Griffith University International Postgraduate Research Scholarship, and Griffith University Postgraduate Research Scholarship (GUPRS) awarded to W.A.O. We acknowledge funding by the National Health and Medical Research Council Fellowship (#1174091) and Program Grant (#1132975), and a Medical Research Future Fund grant awarded to M.F.G.r W.A.O. contributed to study design, planned/conducted all experiments, analyzed/interpreted all data, prepared all figures, and wrote the manuscript. M.-F.H. assisted with setting up initial standard operating procedure for in-house preparation of liposomes, proliferation assay, rodent work, and flow cytometry. M.Z. established initial standard operating procedure for in-house preparation of liposomal formulations. E.C. assisted with rodent work. P.S. assisted with rodent work and FACS staining for B-cell transfer study in mu MT mice. M.B. assisted with FACS staining for CD8<SUP>+</SUP> T-cell and B-cell transfer studies. M.W. assisted with vaccinations for the s.c. versus i.m. study. R.J.N. assisted with harvesting and processing spleens for CD4<SUP>+</SUP> T-cell purification and transfer study. L.B. and D.M. assisted with immunohistochemistry assay. D.I.S. assisted with proliferation assay, oversaw study design, and contributed to the editing of the manuscript. M.F.G. oversaw overall study design and interpretation of the data and contributed to the writing of the manuscript.